Category Archives: Data analysis

Tutorial: bulk segregant analysis in yeast

This is a tutorial on how to do Bulk Segregant Analysis (BSA) in yeast, a particular way of doing Quantitative Trait Locus (QTL) mapping. The general aim is to identify the mutations underlying a trait difference between individuals–in this case, different yeast strains. … Continue reading

Posted in Data analysis, Microbial physiology | 4 Comments

Calculating growth rate from microbial growth curves using MATLAB

Growth curve experiments are used to study the physiology of bacteria, yeast, or other micro-organisms. You inoculate cells in a nutrient medium, let them grow, and record the optical density of the culture over time with a spectrophotometer. Automated plate … Continue reading

Posted in Data analysis, Microbial physiology, Quantitative principles | 1 Comment